GESAN AST-GOT LR

Use

Kit for measurement of aspartate-aminotransferase in serum or plasma – Kinetic UV optimized method IFCC* * International Federation of Clinical Chemistry and Laboratory Medicine

Summary

AST measurements are used in the diagnosis and treatment of certain types of liver and heart disease.

Principle

The enzyme aspartate-aminotransferase (AST) (or glutamic-oxaloacetic transaminase/GOT) catalyzes reaction between alpha-ketoglutarate and L-aspartate giving glutamate and oxaloacetate. In presence of malate dehydrogenase (MDH), oxaloacetate reacts with NADH giving malate and NAD+. The rate of decrease of absorbance due to oxidation of NADH to NAD+ is directly proportional to sample AST activity.

The enzyme aspartate–aminotransferase (AST) (or glutamic–oxaloacetic transaminase/GOT) catalyzes reaction between alpha–ketoglutarate and L–aspartate giving glutamate and oxaloacetate. in presence of malate dehydrogenase (MDH), oxaloacetate reacts with NADH giving malate and NAD+. The rate of decrease of absorbance due to oxidation of NADH to NAD+ is directly proportional to sample AST activity.

Reagents

1. R1
   • Goods buffer pH 7.8 80.0 mmol/l
   • L-aspartate 240.0 mmol/l
   • LDH ≥ 1800 U/l, MDH ≥ 800 U/l
2. R2
   • Goods buffer pH 7.8 80.0 mmol/l
   • alpha-ketoglutarate 65.0 mmol/l
   • NADH ≥ 1.18 mmol/l

Kemenkes RI AKL 20101719340