Use
Kit for measurement of alkaline phospatase in serum or plasma. Colorimetric optimized method DEA DGKC.
Summary
Alkaline phospatase measurments are used in the diagnosis and treatment of hepatobiliary and bone disease.
Principle
Kinetic analysis. Alkaline phosphatase (ALP) catalyzes the hydrolysis, in alkaline environment, of p-nitrophenylphosphate into p-nitrophenol and phosphate. The rate of increase in absorbance due to the formation of p-nitrophenol is directly proportional to sample ALP activity.
Reagents
- R1
- Diethanolamine buffer pH 9.8 3.5 mmol/l
- magnesium chloride 1.0 mmol/l
- R2
- P-nitrophenylphosphate 45.0 mmol/l
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