GESAN Urea UV LR

Use

Kit for measurement of urea in serum, plasma and urine. Kinetic UV method Urease-GLDH

Summary

Urea Increase in serum can be the result of kidney dysfunction or urinary tract obstruction.

Principle

Fixed time analysis. Urea is hydrolysed, in the presence of water, in urease to produce carbon dioxide. The ammonia produced, in the first reaction, combines with alpha-ketoglutarate and NADH which, in the presence of glutamate-dehydrogenase, forms glutamate and NAD+. The decrease in absorbance at 340 nm due to depletion of NADH is proportional to the urea concentration in the sample.

Reagents

1. R1
   • Goods buffer pH 7.6 130.0 mmol/l
   • ADP 1.2 mmol/l
   • Urease ≥ 8000 U/l
   • GLDH ≥ 1500 U/l
2. R2
   • Goods buffer pH 10.2 100.0 mmol/l
   • alpha-ketoglutarate 65.0 mmol/l
   • NADH 1.20 mmol/l

KEMENKES RI AKL 20101717622