VITRO Sepsis Flow Chip Kit

Use:

Sepsis Flow Chip is an in vitro diagnostic kit for human nosocomial infections based on multiplex PCR and reverse dot blot hybridization for simultaneous detection of bacteria, fungi, and major antibiotic resistance genes in a single assay. Sepsis Flow Chip system allows simultaneous detection of over 36 bacteria species (Coagulase-Negative Staphylococci, Staphylococcus aureus, Streptococcus spp., Streptococcus pneumoniae, Streptococcus agalactiae, Streptococcus pyogenes, Listeria monocytogenes, Enterococcus spp., Pseudomonas aeruginosa, Acinetobacter baumannii, Neisseria meningitidis, Stenotrophomonas maltophilia, Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Enterobacteriaceae species, and Proteus/Morganella spp.), fungi species (Candida albicans and Candida spp.)

Principles: 

The Sepsis Flow Chip is based on a methodology which involves the simultaneous amplification of at least 36 bacterial species and several fungal species plus twenty resistance markers by multiplex PCR followed by the hybridization in membranes with DNA specific probes through the DNA-Flow technology for hybriSpot platforms, both automatic and manual. The biotynilated amplicons generated after the PCR are hybridized in membranes containing an array of specific probes for each pathogen and resistance marker, as well as amplification and hybridization control probes. The DNA-Flow technology allows the fast binding of the PCR product and its specific probe in a three-dimensional porous environment, as compared to the hybridization in a conventional surface. Once the binding between the specific amplicons and their corresponding probes has occurred, the signal is visualized through an immunoenzymatic colorimetric reaction with Streptavidin-Phosphatase and a chromogen (NBT-BCIP) generating insoluble precipitates inthe membrane in those positions in which there has been hybridization. The results are analysed automatically with software HybriSoft.

STORAGE AND STABILITY CONDITIONS

Sepsis Flow Chip kit consists of 2 components that are supplied in separate boxes:

• Multiplex PCR Reagents: Shipped at 2-8°C* and then stored at -20°C after reception. Thaw on ice just before use. Reagents are stable until expiry date. These reagents must be stored isolated from any source of contaminating DNA (e.g. PCR products). Avoid freezing and thawing more than five times.
• Hybridization Reagents: Shipped and stored at 2-8°C*. Do not freeze. Reagents and Chips are stable until expiry date.

The hybridization reagent A must be pre-heated in a thermostatized bath or heater (only before using in manual equipment) at 51°C before its use. The rest of the hybridization reagents must be used at room temperature (20-25°C).

VITRO Sepsis Flow Chip Kit (PCR Reagents) KEMENKES RI AKL 20303122606

VITRO Sepsis Flow Chip Kit (Hybridiation Reagents) KEMENKES RI AKL 20303122785